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John A. Ambrose is an American physician who is an expert in coronary artery disease. He is one of the pioneers in acute coronary syndromes having published over 40 articles in the cardiology literature between 1985 and 2000 on their pathogenesis. He has also published on cigarette smoking and cardiovascular disease. Working with his PhD candidate, Rajat Barua, utilizing a novel in vitro model, they described the effects of cigarette smoking on nitric oxide biosynthesis, endothelial function, and endothelial-derived fibrinolytic and antithrombotic factors. Their 2004 update on cigarette smoking and cardiovascular disease published in the Journal of the American College of Cardiology has been referenced over 2,100 times as of 2020. Ambrose is a Professor of Clinical Medicine at the University of California, San Francisco. He was also a Director of the Cardiac Catheterization Laboratory at Mount Sinai Hospital and received a National Leadership Award from the National Republican Congressional Committee.