fluorescamine

Chemical compound

Chemical compoundtype of chemical entityQ238913

Formula: C₁₇H₁₀O₄
1
Sources
1.Stated in PubChem“Fluorescamine”Retrieved 2016-10-14
View statement on Wikidata ↗
Mass: 278.058 Da
1
Sources
1.Stated in PubChem“Fluorescamine”Retrieved 2016-10-14
View statement on Wikidata ↗
Melting point: 157°C
1
Sources
1.Stated in Jean-Claude Bradley Open Melting Point Dataset
View statement on Wikidata ↗

Overview

From Wikipedia, the free encyclopedia · Read full article · Text CC BY-SA 4.0

Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products, i.e. it is fluorogenic. It hence has been used as a reagent for the detection of amines and peptides. 1-100 μg of protein and down to 10 pg of protein can be detected. Once bound to protein the excitation wavelength is 381 nm and the emission wavelength is 470 nm (blue). This method is found to suffer from high blanks resulting from a high rate of hydrolysis due to requiring a large excess concentration. Alternative methods are based on ortho-phthalaldehyde (OPA), Ellman's reagent (DTNB), or epicocconone.

Overview

Chemistry

Computed by PubChem CID 37927 (NIH) · Public domain

Molecular formula: C17H10O4
Molecular weight: 278.26 g/mol
IUPAC name: 4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
SMILES: C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
InChIKey: ZFKJVJIDPQDDFY-UHFFFAOYSA-N
XLogP: 2.9
Topological polar surface area: 52.6 Å²
H-bond donors / acceptors: 0 / 4
Complexity: 503

Chemistry

All data

29 statements · 52% with external sources · numbers in brackets are citations

Canonical SMILES: C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
1
Sources
1.Stated in PubChem“Fluorescamine”Retrieved 2016-10-14
View statement on Wikidata ↗
Subclass of: chemical compound
Has use: indicators and reagents
1
Sources
1.Stated in Medical Subject HeadingsRetrieved 2018-03-15
View statement on Wikidata ↗

Data quality

statements

52%

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Source last edited November 1, 2026. AIDb refreshes this page daily.

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AIDb. (2026). fluorescamine (Q238913). Retrieved from https://aidb.si/e/fluorescamine-Q238913